From Chris.Diment from SESIAHS.HEALTH.NSW.GOV.AU Tue Aug 5 01:24:13 2008 From: Chris.Diment from SESIAHS.HEALTH.NSW.GOV.AU (Chris Diment) Date: Tue Aug 5 11:14:08 2008 Subject: [Immunology] Latex Allergy Message-ID: <0EA4DB4838D3E84B882F62F100D6C423233490@sesiahs.health.nsw.gov.au> Is it possible for you to let me have any information that you have for latex allergy up to 1996. I would really like 1995 and before if that is possible. If you can't provide can you please tell we where I could find this information and if it was availble to the Australian Health Department. I know I am being painful and I am sorry, but I just don't know where the accurate information is. Many thanks, Chris Diment --------------------------------------------------------------------------------------------- SOUTH EASTERN SYDNEY AND ILLAWARRA AREA HEALTH SERVICE CONFIDENTIALITY NOTICE NB: *** Due to an organisational amalgamation, email addresses for recipients in this organisation have changed. Please update your contacts list with the details of the email addresses contained within. This email, and the files transmitted with it, are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, you are not permitted to distribute or use this email or any of its attachments in any way. We also request that you advise the sender of the incorrect addressing. This email message has been virus-scanned. Although no computer viruses were detected, South Eastern Sydney and Illawarra Area Health Service accept no liability for any consequential damage resulting from email containing any computer viruses. From help.you.i.can from gmail.com Mon Aug 11 04:29:36 2008 From: help.you.i.can from gmail.com (SanHolo) Date: Mon Aug 11 11:15:23 2008 Subject: [Immunology] Re: Latex Allergy References: Message-ID: Well, you could search PubMed (http://www.ncbi.nlm.nih.gov/sites/ entrez) for "Latex allergy" and look for articles older than 1996. You can sort by publish date and then jump back some pages to find the older ones. From phanlon from Standardprocess.com Wed Aug 20 10:57:22 2008 From: phanlon from Standardprocess.com (Hanlon, Paul) Date: Thu Aug 21 11:59:09 2008 Subject: [Immunology] Adding apoptosis marker to cell cycle analysis Message-ID: <5C151339422DFD45AA750777EB6B4EB40419E997@spiexchange01.standardprocess.com> I have been conducting research on the effects of phytochemicals and cell cycle and apoptosis and I would like to find a way to run both assays on the same cells. I am currently using the NIEHS protocol for measuring cell cycle with PI and an anti-BrdU FITC antibody. I would like to keep this method since I really like the added confidence of S phase determination that the BrdU tagging gives me. I am looking for an apoptosis marker to add to this, does anyone have anything they know or would hypothesize would work? I'm thinking either a caspase substrate or some sort of antibody to an apoptosis-related protein. My current protocol gives me excellent cell cycle staining and it involves: 1. Adding BrdU to the cells for 40 minutes 2. Fixing the cells with ethanol 3. Solubilizing the cells with 2N HCl + 0.5% Triton-X 100 4. Overnight staining with the anti-BrdU antibody (BD) 5. Overnight staining with PI I then read on the flow with a FACSCalibur (FL1 = FITC, FL3 = PI). I'm willing to try another DNA stain if it helps to free up a detector, but I'd really like to leave the rest of the method the same because it is working so well. I'd like to add something like a caspase substrate which I could incubate at the same time as the BrdU, but I'm afraid of the substrate disappearing when the cells are solublized. I'm willing to try anything, so educated guesses would be appreciated. Thanks Paul Hanlon Manager of Discovery Science Standard Process, Inc. 1200 West Royal Lee Dr. Palmyra, WI 53156 Phone: 262-495-6493 Fax: 262-495-6399 phanlon@standardprocess.com From shauke from uni-bremen.de Wed Aug 27 03:31:31 2008 From: shauke from uni-bremen.de (Bluescript) Date: Wed Aug 27 12:30:34 2008 Subject: [Immunology] Counterstain for AEC AND BCIP/NBT Message-ID: <1db412de-91ea-47d6-8fdc-89cb89ea1c43@x35g2000hsb.googlegroups.com> Hi, I want to do immunohistochemistry detecting two targets at the same time. For various reasons I am going to use AEC as substrate for HRP and BCIP/NBT as AP substrate. Now I am looking for a good counterstain. Because of the AEC the counterstain has to work with an aqueous embedding medium. Has anybody a good idea? Thank you very much for your help in advance, Sven From dkantar from assumption.edu Thu Aug 28 17:35:14 2008 From: dkantar from assumption.edu (Kantarelis, Demetrius (Economics)) Date: Fri Aug 29 10:08:45 2008 Subject: [Immunology] FW: Acapulco and Hawaii 2009 Immunology Conferences Message-ID: Acapulco/Mexico & Hawaii/USA 2009 Immunology Conferences CALL FOR ABSTRACTS / PAPERS / POSTERS: The Frontiers in Immunology Research 2009 Conferences will be held in Acapulco, Mexico (January 8-11 at the Hyatt Regency Hotel) and in Hawaii (Kona), USA (July 22-26 at the Sheraton Hotel.) You may participate as panel organizer, presenter of one or two papers or posters or as an observer. The deadlines for abstract / poster submissions and registration are: September 30th, 2008 (Acapulco) and March 30th, 2009 (Hawaii). For more information, please contact the Frontiers in Immunology Research Network (FIRN) as follows: FIRN, 64 Holden Street, Worcester, MA 01605-3109, USA. Telephone: 508-852-3937, Fax: 508-595-0089, Email: