From dkantar from assumption.edu Sun Mar 2 14:15:34 2008 From: dkantar from assumption.edu (Kantarelis, Demetrius (Economics)) Date: Sun Mar 2 16:30:15 2008 Subject: [Immunology] Reminder: Deadline, March 30th, 2008 / Florence Immunology Conference References: Message-ID: FRONTIERS IN IMMUNOLOGY RESEARCH 2008 INTERNATIONAL CONFERENCE www.firnweb.com FLORENCE, ITALY July 22-26, 2008 Hotel Croce di Malta The Frontiers in Immunology Research Network (FIRN) invites you to participate in its 2008 Conference to be held in the Florence, Italy, at the Croce di Malta Hotel, July 22-26. The conference welcomes researchers from academia, corporations, governments and other organizations. Participants may present their research findings, display their posters, participate in roundtables or simply observe. To participate as a PRESENTER please submit via email and post ABSTRACTS and/or brief descriptions of POSTERS (of no more than 200 words) along with your complete details by March 30th, 2008 as follows: Frontiers in Immunology Research Network (FIRN) 64 Holden Street Worcester MA 01605-3109 USA Tel.: 508-852-3937, Fax: 508-595-0089 Email: hkan@firnweb.com Web: http://www.firnweb.com From hotsciencegeek from live.com Tue Mar 4 18:24:42 2008 From: hotsciencegeek from live.com (Elena Sartorius) Date: Wed Mar 5 11:39:13 2008 Subject: [Immunology] T-cell proliferation suggestion! Message-ID: Hi N K,With regards to the proliferation assay, i would suggest the following -1. for the cd3 concentration, try 25mg/ml. i find this is highly effective2. instead of using DMEM based media, try using human placental blood. while there maybe ethical issues..data is data :)!3. increase your incubation time to one week.By the way, i have realised that there are not many people in this field - specifically in T-cell proliferation in mice.Would you like to meet for a drink sometime and talk science or more?? with warm regards,Elena Sartorius Im trying to do a mouse T cell proliferation assay. According to the literature out there one should be able to induce CD4+ T cell proliferation by using anti cd3 and anti cd28. But I cant seem to get this to work...i.e. the cells are not multiplying (im measuring proliferation by using Brdu and FACS). I have tried different concentrations for cd3 (from 3ug/ml to 25 ug/ml) and cd28 (2ug/ml to 8ug/ml). I also tried them as both platebound and soluble antibodies. I dont know what I am doing wrong...any help/suggestions or protocols would be greatly appreciated! Thank You N K _________________________________________________________________ Helping your favorite cause is as easy as instant messaging.?You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join From dkantar from assumption.edu Mon Mar 17 13:27:46 2008 From: dkantar from assumption.edu (Kantarelis, Demetrius (Economics)) Date: Mon Mar 17 13:33:23 2008 Subject: [Immunology] FW: Florennce / Italy Immunology Conference/Deadline Reminder: March 30th, 2008 Message-ID: Deadline Reminder for submission of abstract and poster: March 30th, 2008 FRONTIERS IN IMMUNOLOGY RESEARCH 2008 INTERNATIONAL CONFERENCE www.firnweb.com FLORENCE, ITALY July 22-26, 2008 Hotel Croce di Malta The Frontiers in Immunology Research Network (FIRN) invites you to participate in its 2008 Conference to be held in the Florence, Italy, at the Croce di Malta Hotel, July 22-26. The conference welcomes researchers from academia, corporations, governments and other organizations. Participants may present their research findings, display their posters, participate in roundtables or simply observe. To participate as a PRESENTER please submit via email and post ABSTRACTS and/or brief descriptions of POSTERS (of no more than 200 words) along with your complete details by March 30th, 2008 as follows: Frontiers in Immunology Research Network (FIRN) 64 Holden Street Worcester MA 01605-3109 USA Tel.: 508-852-3937, Fax: 508-595-0089 Email: hkan@firnweb.com Web: http://www.firnweb.com From dkantar from assumption.edu Tue Mar 18 13:45:43 2008 From: dkantar from assumption.edu (Kantarelis, Demetrius (Economics)) Date: Tue Mar 18 17:47:02 2008 Subject: [Immunology] Florence Immunology Conference: Deadline: March 30th, 2008 References: <200803181703.m2IH3aL04512@net.bio.net> Message-ID: Florence Immunology Conference: Deadline: March 30th, 2008 FRONTIERS IN IMMUNOLOGY RESEARCH 2008 INTERNATIONAL CONFERENCE www.firnweb.com FLORENCE, ITALY July 22-26, 2008 Hotel Croce di Malta The Frontiers in Immunology Research Network (FIRN) invites you to participate in its 2008 Conference to be held in the Florence, Italy, at the Croce di Malta Hotel, July 22-26. The conference welcomes researchers from academia, corporations, governments and other organizations. Participants may present their research findings, display their posters, participate in roundtables or simply observe. To participate as a PRESENTER please submit via email and post ABSTRACTS and/or brief descriptions of POSTERS (of no more than 200 words) along with your complete details by March 30th, 2008 as follows: Frontiers in Immunology Research Network (FIRN) 64 Holden Street Worcester MA 01605-3109 USA Tel.: 508-852-3937, Fax: 508-595-0089 Email: hkan@firnweb.com Web: http://www.firnweb.com