Hi,
There are many anticogulants e.g haparin, ACD and EDTA are few of them.Usage of ACD/EDTA/heparin actually depends what you want to do with isolated neutrophiles.
In our experience of DNA typing, we are using ACD instead of EDTA. Acid Citrate Dextros has advantage especially in DNA amplification (PCR reactions) as EDTA cause amplification problem in amplification, and Heparin is expensive.
with regards
Raza Ali
Scientific research officer
SIUT.
> Date: Thu, 7 May 2009 12:04:00 -0500
> From: immuno-request from oat.bio.indiana.edu> Subject: Immuno Digest, Vol 45, Issue 1
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>> 1. Human Peripheral blood's Neutrophil isolation (Faimola Guerrero)
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> Date: Wed, 06 May 2009 12:25:02 -0500
> From: Faimola Guerrero <fguerrero from biothera.com>
> Subject: [Immunology] Human Peripheral blood's Neutrophil isolation
> To: <immuno from magpie.bio.indiana.edu>
> Message-ID: <C627321E.A0%fguerrero from biothera.com>
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>> Hi I am trying to use your protocol to isolate neutrophils. What is the
> purpose of the ACD is it a substitute for heparin?
>> --
> Faimola Guerrero
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