IUBio

[Immunology] Question about NBT assay?

Luis Antonio Ochoa Ramírez via immuno%40net.bio.net (by ant127 from hotmail.com)
Tue Jun 29 15:30:27 EST 2010





Hi,
 
I'm trying to perform a NBT assay to measure O2 production from monocytes. I don't have much experience in this but so far I know that I need a NBT saturated solution + PMA. My question is: what concentration of those reagents should I use? 
 
About the solvent, I have considered two options: RPMI 1640 + 10% FCS or Hank's balanced salt solution (+Ca, +Mg), but I haven't decided.
 
By the way, it's for a colorimetric test. 
 
Any help about one or both of my questions would be really appreciated.
 
Thanks   


Biol. Luis Antonio Ochoa Ramírez





Soy como quiero ser en mi Messenger



En Messenger están mis contactos con los que sí tengo contacto



¿Te falta espacio para tus correos? Enciende tu hotness con Hotmail
 		 	   		  
_________________________________________________________________
Hotmail: as HOT as always
www.hotmailhotness.com.mx


More information about the Immuno mailing list

Send comments to us at biosci-help [At] net.bio.net