Dear Bryan,
I am using your protocol for Neutrophiles isolation:
http://www.bio.net/-archive/immuno/2001-August/016695.html
I just needed a fast method to eliminate erythrocytes and it works
finely to me. I am trying to set up a MALDI-MS method of lipid analysis
for the screening of Barth Syndrome. (Briefly) It is a rare disease
caused by the lack of cardiolipin in mitochondria, so I had to eliminate
erythrocytes because they do not have mitochondria. I have two questions
for you:
1- Are you sure that the hypotonic lysis step eliminates also platelets?
2- Can you suggest me a paper of yours where this protocol has been used
so I can cite it?
Best regards and many thanks!
--
Roberto Angelini
PhD
Università degli Studi di Bari.
Dipartimento di Scienze Mediche di Base, Neuroscienze e organi di senso
P.za G. Cesare, 70124, Bari, ITALY
tel +39 0805448504
---
Questa e-mail è priva di virus e malware perché è attiva la protezione avast! Antivirus.
http://www.avast.com