Protein purification using mouse anti-Myc Tag IgG

[Nick Theodorakis]

Breslauer wrote:
> Protein purification using mouse anti-Myc Tag IgG
> Hi there,
>
> have question about publications & your experience. I'm going to purify
> partially purifed recombinant protein (but containing ~30% of protein
> contaminations) using affinity chromatography. I produced a lot of
> monoclonal antibody - mouse anti-myc, and my protein has Myc-Tag.
> Saying produced I mean collected supernatant from hybrydoma cells. The
> thing is to purify & bind my antibodies and (it would be great if using
> the same resign/column) pass my protein throuh it, hoping it will bind
> with antibodies. Then wash & elute it. Do u thing that sepharose with
> protein A will be good? Does any of u who have expereince in this
> experiment may discuss this idea of expriment with me? Waiting for your
> replay.

Is your culture supernatant from serum-free media, or does it contain a
lot of bovine anitbodies?

Nick

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Nick Theodorakis
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