monocyte transfection

[DanSolo]

StewJW wrote:
> This protocol is taken from Invitrogen's web site for transfection of
> THP-1 human monocytes.  Its uses:
>

I think you might find a big difference between primary monocytes and
THP1 cells. All transformed lines are faster growing and have weaker
membranes -> easier to transfect. Electroporation is often the only
answer, but it can kill a lot of your cells.