QRTPCR calculations

Ramakrishnan, Rajesh ramakris at bcm.tmc.edu
Sun Sep 3 18:59:40 EST 2006


Hi
Can someone explain how data is analyzed and presented for QRTPCR after ChIP assay. I have the Ct values and have been using the delta delta Ct method to calculate fold changes. How does the Ct value of input DNA figure into the calculation so the data can be presented as percent input. Any help
with the logic and calculations is appreciated.
Thanks
Raj


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