HELP T7 phage needed
Wojtek
wojtek777 at poczta.fm
Mon Sep 11 04:20:12 EST 2006
Hi,
I have a question. I have an expression plasmid (with His tag)
in which the fusion protein gene is under T7 phage promoter.
To express the protein I will need to infect transformed E. coli
with T7 phage? Am I right? Then, where to get T7 phage from?
I checked several companies and didn't find the one selling
T7 phage. Which company sell it? Or, maybe, you get the expresion
some other way? Thanks in advance for help.
Krzysztof
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