question about making phenol/TE and

[Jose de las Heras]

"Christian Praetorius" <prae at gmx.net> wrote in message 
news:4n4vvqF8j66hU1 at individual.net...
> Zhiqun Li <li at lifesci.ucsb.edu> wrote:
>
>>I also tried to make phenol/water saturated buffer in the same time, the
>>aqueous phase is always staying below or close to 5, but not 5. I wonder
>
> Sorry, but why are you making this solution by yourself? This is
> something, that makes sense for a lot of buffers, but Phenol and
> Phenol/Chloroform are not along these buffers. Phenol is quite toxic,
> so buy this buffers in good quality from the usual suppliers.
>
> Christian

I would second that.

Jose