question about making phenol/TE and

[ChenHA]

On Mon, 18 Sep 2006 01:01:04 GMT, dk at no.email.thankstospam.net (DK)
wrote:

>Point taken but I've *never* seen a pre-cast gel that was of higher 
>quality than anything I've poured myself. I've seen plenty of 
>atrocious pre-cast gels though. 

Try Nupage Novex midigel.  It works well, and I can actually see
peptides well separated and which failed to show previously in
home-made tris-tricine gelsl as well as another precast gel from
another company.

But for proteins in general, I use gel I made myself.


> In the past four years, I had a gel 
>leaking exactly one time (and my gel rig is 11 years old original 
>Bio-Rad mini which does have its design problems).
>
>That said, I'd happily use precast gels for something that does not 
>require quality (fraction analysis, purity assessment, etc) if they cost 
>3X less than they are now. At $10/gel, when a lab runs on 
>average ~ 6 gels a day, is a bit too much. 
>
>Obviously there is always a fine balance between saving time and 
>saving money. Ultimately, it comes to down to whether one believes
>that all lab work is "lab monkey" type. 
>
>DK