DNA methylation identification

[Tom Knight]

agen agen <agen_tec at yahoo.co.uk> writes:
> I wonder if anyone out there knows how to identify if the genomic
> DNA is methylated or not apart from the HpaI/MspI digestion ?

The standard approach is to do a bisulfite conversion of the
unmethylated cytosine to uracil, followed by PCR and direct
sequencing, or by PCR, cloning, and sequencing.  This won't work to
analyze adenosine modifications.