how to crosslink dna binding proteins?

Yuval Ebenstein uv at chem.ucla.edu
Fri Sep 29 23:11:38 EST 2006


Hi all,
First let me apologize if i'm not using the right terminology. I'm a 
physical chemist doing his first steps in biology....
I would like to react CAP protein to a piece of DNA with a specific 
binding site, and then cross link and purify so that  I can deposit it 
on a surface and image it with an Atomic Force Microscope (AFM).
Can someone point out a simple but efficient crosslinking and 
purification method?
Thanks.
Yuval

-- 
--------------------------------------------
Yuval Ebenstein
Department of Chemistry and Biochemistry, UCLA
Young Hall-2002, 607 Charles E. Young Drive East
Los Angeles, CA 90095-1569
Ph: (310) 794-6685 (lab)/825-0024(office); Fax: (310) 267-4672



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