(no subject)

[Inge Seim]

Have you tried Googling it ?  Took me about five seconds to find it
http://www.stratagene.com/lit/showVector.aspx?vecId=198

Maybe you did not find it due to
http://news.com.com/Google+to+censor+China+Web+searches/2100-1028_3-6030784.html

I do not know :/

All the Best,
IS

---- Original message ----
>Date: Wed, 20 Sep 2006 12:01:45 -0500 (EST)
>From: methods-request at oat.bio.indiana.edu  
>Subject: Methods Digest, Vol 16, Issue 18  
>To: methods at magpie.bio.indiana.edu
>
>Send Methods mailing list submissions to
>	methods at net.bio.net
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>When replying, please edit your Subject line so it is more specific
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>
>Today's Topics:
>
>   1. NEED A PROTOCOL FOR DNA ISOLATION FROM RICE SEEDLING::: Urea
>      DNA	isolation (LUCKRING, ABIGAIL K.)
>   2. FABA --- search thousands of arrays (czhu at changbioscience.com)
>   3. question about making phenol/TE and (Tim Fitzwater)
>   4. Ask for help! (=?gb2312?B?xuvM7NH0?=)
>   5. Substitute for Corex tubes? (bgreuel at jbu.edu)
>   6. No DNA digestion (Ranadheer Kumar Gupta)
>
>
>----------------------------------------------------------------------
>
>Message: 1
>Date: Tue, 19 Sep 2006 11:22:47 -0400
>From: "LUCKRING, ABIGAIL K." <abigail.k.luckring at cgr.dupont.com>
>Subject: NEED A PROTOCOL FOR DNA ISOLATION FROM RICE SEEDLING::: Urea
>	DNA	isolation
>To: <methods at magpie.bio.indiana.edu>
>Message-ID:
>	<D7CB9B5201050E45A8BEEA45A30F8A3EA543E4 at e320ms02.phibred.com>
>Content-Type: text/plain;	charset="iso-8859-1"
>
>Does anyone know where to get the literature reference for the Urea DNA extraction protocol listed?
>
>Abby Luckring
>(302) 695-4634
>
>
>
>------------------------------
>
>Message: 2
>Date: 19 Sep 2006 08:39:43 -0700
>From: czhu at changbioscience.com
>Subject: FABA --- search thousands of arrays
>To: methods at net.bio.net
>Message-ID: <mailman.1092.1158681494.20007.bio-soft at net.bio.net>
>Content-Type: text/plain; charset="iso-8859-1"
>
>A new version of FABA (Functional Analysis By Association) for
>searching thousands of arrays is available at
>
>http://www.changbioscience.com/faba/
>
>Comments and suggestions will be appreciated!
>
>Thanks.
>
>Chang
>
>
>
>------------------------------
>
>Message: 3
>Date: Tue, 19 Sep 2006 11:14:19 -0600
>From: "Tim Fitzwater" <tfitzwater at somalogic.com>
>Subject: question about making phenol/TE and
>To: <methods at magpie.bio.indiana.edu>
>Message-ID: <771BF0D090CE73449F7495D31E0BA0D101E58B at ELM.sladmin.com>
>Content-Type: text/plain;	charset="iso-8859-1"
>
>Phenol may contain 0.15% H3PO2 as a preservative which may liberate spontaneously flammable phosphine when distilled.  Distill only under an inert atmosphere.  Phenol becomes explosive when heated above 79°C.
>
>Oxidized phenol is pink to red.  The oxidation products are quinones, a wide variety of phenolic coupling compounds and diacids.  Quinones are a, b-unsaturated ketones that are capable of reacting with primary amines.  The reaction of quinones with multiple amines can form stable crosslinked products.  Quinones can crosslink nucleic acids and may break down phosphodiester bonds.  The diacids arise from further oxidation of orthoquinone, while phenolic coupling products result from phenoxide radicals.  Quinones are faintly yellow.  Phenolic coupling reaction compounds are intense yellow or red.     
>0.1% 8-Hydroxyquinoline can be used as an additional preservative; this also turns the phenol layer yellow, making separation of phases easier.  
>Some protocols add 70 mL m-cresol/500 g phenol as antifreeze and as an additional deproteinizing agent.  The m-cresol should be colorless.
>
>Phenol is most stable as crystals at -20°C, but is most practically stored in a liquid-saturated form at 4°C.  The aqueous phase of water-saturated phenol is typically pH 3.0-4.5.  The low pH is due to partitioning of the diacids into the aqueous phase.  Partitioning will proceed more rapidly in a buffered solution of a higher pH, however, phenol has a pKa = 10.0 and is much more susceptible to oxidation at high pH.  Buffers used to equilibrate phenol should not exceed pH 8.0.  
>
>There is a wide variation in the amount of NaOH or Tris buffer required to adjust the pH of phenol, which makes purchase of commercial versions very attractive and highly recommended.
>
>To measure the pH of phenol:
>Organics such as phenol and chloroform have much lower dielectric constants than water.  The very large liquid junction potential can cause problems such as pH drift, long stabilization times and pH electrode damage.  The indicator chemical in pH paper is destroyed by phenol resulting in inaccurate pH measurement.  In order to accurately measure the pH of saturated phenol it is necessary to solubolize the phenol in an aqueous medium. 
>
>For saturated phenol (no chloroform), mix 2 mL organic phase with 5 mL methanol and 13 mL water and pH entire sample.  pH will drift down over the course of about 30 seconds until it stabilizes.  (This method is from the Ambion 1994 catalog.)  
>
>For phenol:chloroform or acid phenol:chloroform, mix 2 mL of the organic phase with 8 mL methanol and 10 mL water.  Mix and measure pH of the entire sample with the pH electrode.  pH will drift down over the course of about 30 seconds until it stabilizes. 
>
>Silver/silver chloride type electrodes are not recommended because they do not accurately measure the pH of Tris.  Since the electrode dehydrates during phenol testing, it should be soaked in Type I water to rejuvenate the glass bulb.
>
>
>
>
>
>
>------------------------------
>
>Message: 4
>Date: Wed, 20 Sep 2006 10:18:55 +0800
>From: "=?gb2312?B?xuvM7NH0?=" <qity836 at nenu.edu.cn>
>Subject: Ask for help!
>To: methods at magpie.bio.indiana.edu
>Message-ID: <358718735.24812 at nenu.edu.cn>
>Content-Type: text/plain
>
>
>I am looking for the restriction map of plasmid named pBS for a long time.
>Look forward to your reply.Thank you very much!
>
>sincerely yours,
>
>Qitianyang.
>Institute of Genetics and Cytology
>Northeast Normal University
>changchun 130024 CHINA
>
>
>
>
>
>
>------------------------------
>
>Message: 5
>Date: 19 Sep 2006 22:41:20 -0700
>From: bgreuel at jbu.edu
>Subject: Substitute for Corex tubes?
>To: methods at net.bio.net
>Message-ID: <1158730880.424968.57940 at k70g2000cwa.googlegroups.com>
>Content-Type: text/plain; charset="iso-8859-1"
>
>Apparently the 30 ml glass Corex tubes have not been available for
>several years now.  What other brands of glass centrifuge tubes are
>good substitutes for these tubes and where have you purchased them?
>Thanks for your assistance.
>
>Brian
>
>
>
>------------------------------
>
>Message: 6
>Date: Wed, 20 Sep 2006 18:13:35 +0530
>From: "Ranadheer Kumar Gupta" <ranadheergupta at gmail.com>
>Subject: No DNA digestion
>To: Methods at magpie.bio.indiana.edu
>Message-ID:
>	<20340af40609200543q2dc16519wd42784e1eb8ec5aa at mail.gmail.com>
>Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
>Hi all,
>i have a problem in digesting the genomic DNA from Leucaena
>leucocephala. i tested the restriction enzymes, they are working on
>other DNA but not on this plant genomic DNA. i followed CTAB method
>for DNA isolation. Is it because of polysacharide contamination?
>please sujjest me
>
>thanking you all
>
>Ranadheer
>
>
>
>------------------------------
>
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