co-existence of expression vector and promoterless reporter vector in the same cells

[Aawara Chowdhury]

In <mailman.624.1175713225.5139.methods from net.bio.net>,
 waikhay <waikhay from gmail.com> wrote:

> Hi all,
>
> I am currently working on cloning and expressing a bacterial regulatory
> protein using an expression vector to test for its binding to a bacterial
> promoter which is cloned into a promoterless reporter vector that can
> ideally co-exist with the expression vector in the same E. coli cells.
> However, the expression vectors (e.g. pQE seris by Qiagen and pET series by
> Novagen) and the promoterless reporter vectors (e.g. pZsGreen1-1 and
> pDsRed-Express-1 by Clontech) that I found share the origin of replication
> which falls into the same plasmid incompatibility group. Does anyone out
> there have an idea of which expression and promoterless reporter vectors I
> can use that can also co-exist in the same cell? Any form of help would be
> greatly appreciated!!

pET and pQE vectors have derivatives of the ColE1 origin.  Your reporter
plasmid can have the pSC101 origin, as it lies in a different incompatibility
group.

AC
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