tyrosinase immobilization?

StewJW via methods%40net.bio.net (by stewjw from gmail.com)
Wed Apr 11 03:33:32 EST 2007


GE Healthcare (formely Pharmacia products) have a large range of
column matrices for protein purification:

http://www4.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&ParentId=702515

Also, check out Pierce which have some nice kits:

http://www.piercenet.com/products/browse.cfm?fldID=020504

and if you have don't have the chromtaography kit check out magnetic
beads, you just need a relatively cheap magnetic base for tubes.
Invitrogen sell Dynal surface activated magnetic beads:

https://catalog.invitrogen.com/index.cfm?fuseaction=viewCatalog.viewCategories&pc=1011&npc=92&nc=971&

The epoxy beads would give you a stable linkage to amino groups on
your protein. There are two sizes of beads , the larger beads are
designed for large proteins such as antibodies.



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