Western blotting problem

[Pow Joshi]

On 4/20/07, smak from parkinsonsinstitute.org <smak from parkinsonsinstitute.org> wrote:
> Hi,
>
>
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> I have some problems on running the SDS PAGE. I found that the western
> blot shows higher level of detection on one side and moving gradually
> lower to another side. I use the Biorad wet transfer unit at the same
> loading of the total proteins.

do you mean that you can detect high molecular weight proteins better
than the low mol weight ones? or the other way round?

>
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> Thanks a lot,
>
> Sally
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