How to identify lanes on large agarose gels?

[Michael Sullivan]

I don't see how it would have an impact on the bands you are  
interested in. I would think just adding some linear plasmid to your  
sample buffer so that you get a concentration of say 20 ng/lane would  
work nicely.

Mike

On Aug 6, 2007, at 5:11 PM, Peter Frank wrote:

> Michael Sullivan <mlsulliv from wisc.edu> wrote:
>
>> Why not include something bigger in size than you expect for your PCR
>> products in every lane, e.g. linerarized plasmid.
>
> Yes, that would probably work. Do you think that would impair the
> samples somehow? If yes, how about adding that plasmid  into each well
> shortly before I stop the gel run so that the linearized plasmid would
> only run by itself and only a small distance into the gel?
>
> Peter
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