RR to DK: How to efficiently transfect U937 cells with a plasmid vectorexpressing shRNA

[Prof. Piero Sestili]

Daer DK,

that's exactly what I thought...a method is a method and cannot do more
than it does...  As to electroporation, that we have already tried, and
as I wrote in my first SOS, we're particularly concerned about the
overall viability of electroporated cultures. Maybe your guide might be
helpful at this specific regard...Thanks a lot in advance


Piero

Prof. Piero Sestili
Istituto di Farmacologia e Farmacognosia e
Centro di Ricerca sull'Attività Motoria
Università degli Studi di Urbino "Carlo Bo"
Via "I Maggetti" 26
61029 URBINO (PU)
Tel. 0722 303414; 0722 305524 
Fax 0722 303401


-----Messaggio originale-----
Da: methods-bounces from oat.bio.indiana.edu
[mailto:methods-bounces from oat.bio.indiana.edu] Per conto di DK
Inviato: mercoledì 8 agosto 2007 23.48
A: methods from magpie.bio.indiana.edu
Oggetto: Re: R to DK: How to efficiently transfect U937 cells with a
plasmid vectorexpressing shRNA

In article <mailman.562.1186581864.11350.methods from net.bio.net>, "Prof.
Piero Sestili" <piero.sestili from uniurb.it> wrote:
>Thanks a lot DK,  
>
>we have something like this guide but there's nothing "amazing"....so
>yes, please send your one to the public address or, if you prefer to
>piero.sestili from uniurb.it. Hi and thanks again

I promise, there isn't anything *amazing* in my guide either :-)
Mostly common sense and explanation if how various factors 
come into play. 

OK, give me some time to find it and maybe update some parts. 

DK
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