His Tag antibody

[Josh Levin]

On Aug 19, 1:33 pm, Ozan Aygun <metugenet... from yahoo.com> wrote:
> Hi,
>
> I am afraid at least refering to the experience with
> HIS epitope tag in my hands, I would say it is one of
> the worst choices for the mammalian system.
>
> His antibodies give a lot of background in the whole
> cell extracts of mammalian cells probably due to the
> presence of histidine rich proteins. Therefore one
> should either make a very stringent washing or simply
> play around the dillution of the antibody.
>
> A partially purified extract may give somehow better
> results.
>
> For IF aplications and for more specificity, I suggest
> you use myc, HA or FLAG epitopes for tagging instead
> of HIS in mammalian cells.
>
> Hope this may be any help, good luck.
>
> Ozan
>
> ____________________________________________________________________________________
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Our company sells a product called "HisDetector"-- it is an
alternative to an anti-His antibody that has a nickel-NTA group
covalently bound to an HRP or AP detection enzyme.    See www.kpl.com
for more info.

Josh Levin
KPL, Inc.