[Western Blotting] Diffusion of Antibodies in PVDF and NZ membranes

[lowenbrau]

> newsnet customer wrote:
>>> newsnet customer <customer from newsnet.com> wrote:
>>>
>>>> I have experienced protein diffusion from my membrane after an 
>>>> overnight
>>>> antibody incubation. This was confirmed with a positive control. And 
>>>> since
>>>> he had no signal or reduced signal on his membrane, this could be a 
>>>> possible
>>>> explanation. Please realise that comments on a newsgroup should be 
>>>> taken
>>>> with a grain of salt. And you should take a chill pill.
>>> So, as Kyle suggested, you are a troll.  If your technique in the lab is
>>> as thorough as your knowledge, there are better explanations for your
>>> failed Western.
>>
>> Can you read troll? As confirmed my western failed because of the long 
>> incubation time. However, i'm curious to hear some of your "better 
>> explanations" especially when you don't know the details of my experiment 
>> or the experiment of the original post. Tool.
>>
> I'm willing to bet you tried to blot your protein to nylon.

Many people do so whats ur point? and don't tell me that PVDF doesn't have 
this problem cos ur a tool.

ST