Simple question that is hard to answer

[ChenHA]

On Thu, 15 Feb 2007 16:51:49 -0500, "Carlo Zambonelli"
<zambonel from gmail.com> wrote:

>Yes, as long as you have 2 different selective markers and the appropriate
>cell strain. See http://www.emdbiosciences.com/html/NVG/DuetTable.html
>C.
>

I think you misunderstood.  The Duet series are plasmids with
different replicons, so are different as far as the original question
is concerned.  The question would be one about plasmids with the same
replicon, and that is problematic.  If there are different antibiotics
used, then it may be possible to maintain the plasmids, but do
remember that antibiotics like ampicillin are broken down fairly
quickly, and when the antibiotics are gone, there is in fact no
selection, and one of the plasmids will most probably be lost.

I would suggest trying different plasmids for the experiment.  The
Duet series would be a useful one since they are of different
replicons, although they are designed for expressing multiple genes (2
in each plasmids).   It is therefore possible to clone both genes into
the same plasmids, although I'm wary of having 2 copies of essentially
the same gene in one plasmids (possible deletion).  It might be
cheaper and easier just to find someone who has a different plasmid,
e.g. a pACYC based plasmids


>On 15 Feb 2007 12:20:34 -0800, peter <peter.ianakiev from gmail.com> wrote:
>>
>> Hi group,
>> I have a question regarding incompatibility of plasmids. Can one
>> bacterial cell be transformed with more than one plasmid (of the same
>> kind) and keep these plasmids. For example if one has two clones (same
>> insert, same vector) can they co-exist in the same cell (same size
>> too, only difference would be point mutation). Your thoughts are
>> invaluable and much appreciated.
>> Peter
>>
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