ccdB insensitive dam/dcm minus E.coli strain

[Tom Knight]

"Josmar" <josmarlangner At yahoo.de> writes:
> to prepare dam/dcm unmethylated DNA of Gateway vector constructs I am
> in need of a dam/dcm minus E.coli strain that is insensitive to ccdB
> either by carrying ccdA or gyrA462. Does anyone know of such a strain
> or made it in the lab?

I don't have the strain, but you might consider just doing PCR of the
vector which will produce unmethylated DNA.  Isn't the ccdB operon
removed during gateway cloning reactions?