Quesion about DNA gel digestion

[Sampath Natarajan]

Dear all,

I am working in the field of DNA cross linking. So I need to confirm the chemical cross linking in DNA with polyacrylamide-urea (DNA gel digestion). Since I don't have any prior experience in that, I need some help regarding running the polyacrylamide-urea gel for DNA cross linking. Also I don't know the exact protocol to run this gel. Please give some idea about this and how much concentration of urea I have to use. If anyone give the full protocol about this DNA gel digestion it would be great for me.

Thanking you,

Yours sincerely,
Sampath


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Dr. N.SAMPATH 
Post Doctoral Fellow
  Department of Molecular & Cell Biology
Samsung Biomedical Research Institute
Sungkyunkwan Univ. School of Medicine
Suwon 440-746, S.Korea
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tel : 82-31-299-6150 & 82-31-299-6155   
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