Cotransformation

[Pow Joshi]

Transfecting both plasmids together has worked rather well for me in the
past, especially with transient transfections ..... you would need to
optimize the concentrations of each .....

best
pow

On 6/4/07, waikhay <waikhay from gmail.com> wrote:
>
> Hi all! :)
>
> I would like to transform 2 plasmids i.e. an expression vector (carrying
> the
> gene of interest) and a reporter vector (into which the promoter of
> interest
> has been cloned) into BL21(DE3) cells. Would you recommend/suggest
> transforming both plasmids into the cells at once (cotransform) or
> transforming one plasmid after another (i.e. transform the reporter vector
> into the cells first, select for transformants, then only transform the
> expression vector and select for cotransformants)?
>
> Thanks much,
> Waikhay
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