DNA/RNA random mutation method?

[Tom Anderson]

On Wed, 13 Jun 2007, DK wrote:

> In article <mailman.1477.1181763428.5139.methods from net.bio.net>, "Z.L. K" <athelas0113 from gmail.com> wrote:
>
> >I would like to generate some random mutations on either DNA/RNA,
> >however, i won't get it with UV mutagenesis method. Does anyone know
> >any good method to generate random mutations? Thanks a lot!

How about hydroxylamine treatment:

http://www-rcf.usc.edu/~forsburg/hydroxyl.html

Or EMS:

http://www.ncbi.nlm.nih.gov/sites/entrez?Db=PubMed&Cmd=ShowDetailView&TermToSearch=15137072

Use of a mutator strain of _E. coli_ is also an option.

> Error-prone PCR. Many manufactures sell appropriate polymerases.

Or, i hear, you can just use the worst normal polymerase you have around
(Taq is fine) and throw in more magnesium, some manganese ions, extra
nucleotides and extra polymerase:

http://www.bio.net/bionet/mm/methods/2000-May/083250.html

Or, for Quebecois style EP-PCR, some alcohol, bien sur:

http://www.ncbi.nlm.nih.gov/sites/entrez?Db=PubMed&Cmd=ShowDetailView&TermToSearch=15585138

tom

-- 
Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT
(t) +44 (20) 76797264   (f) +44 (20) 76797805   (e) thomas.anderson from ucl.ac.uk