Frozen/Freezing Cells

[Pow Joshi]

On 6/21/07, Yvonne Couch <yvonne.couch from dpag.ox.ac.uk> wrote:
>
> I froze some cells yesterday in one of those little portable freezing
> things
> in cell freezing medium and to keep it cool while clearing the hood out I
> put it in the -20.  I then COMPLETELY forgot to take the cells through to
> the -80 and was told about it today.  The person who told me said the
> cells
> will almost definitely be dead.  Any different opinions or should I just
> chuck them because there were about 9 vials and I don't want to waste them
> if I don't have to!



I assume they were in -20 for just the night, and that should be totally
fine .... yes, you should go ahead and place them in -80 and thaw out 1 vial
just in case ....like everyone else suggested.... however, I have placed
cells in -20 ON and does'nt matter too much.
best
pow

Cheers
>
> Yvonne
>
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