DNA in buffer
Jose de las Heras
via methods%40net.bio.net
(by josenet from tiscali.co.uk)
Thu Mar 1 21:16:39 EST 2007
"Blunt Tool" <someone from somewhere.com> wrote in message
news:t5dFh.4699$8U4.3107 from news-server.bigpond.net.au...
> Hi
>
> How long can you store plasmid DNA (~6kb) in TE buffer at -20C without no
> mutations?
>
> ST
Mutations?
You may get some degradation, perhaps, if there's some contaminant
nuclease... but I don't think you'll get mutations.
It's better to store plasmids frozen in TE than as a bacterial stock in
glycerol, where you *may* get mutations.
Jose
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