DNA in buffer

Jose de las Heras via methods%40net.bio.net (by josenet from tiscali.co.uk)
Thu Mar 1 21:16:39 EST 2007


"Blunt Tool" <someone from somewhere.com> wrote in message 
news:t5dFh.4699$8U4.3107 from news-server.bigpond.net.au...
> Hi
>
> How long can you store plasmid DNA (~6kb) in TE buffer at -20C without no 
> mutations?
>
> ST

Mutations?

You may get some degradation, perhaps, if there's some contaminant 
nuclease... but I don't think you'll get mutations.

It's better to store plasmids frozen in TE than as a bacterial stock in 
glycerol, where you *may* get mutations.

Jose 




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