Co-Immunoprecipitation of nuclear receptor

[Nath]

Hi everyone,

I'm presently having problem to do my co-immunoprecipitation. I
immunoprecipitate a nuclear receptor and I'm trying to look at the
proteins that are associated to that receptor. The problem is that,
once actif, my receptor become highly link to DNA, so it become
insoluble and the condition that affectes its DNA-protein interaction
also distroyes the protein-protein interaction with my protein of
interest.  I was wondering if I could crosslink my proteins before the
immunoprecipitation (to have the active form of my receptor) and look
at it after by SDS-Page ? Does the condition of an SDS-page is harsh
enough to break all the protein-protein interaction of crosslink
proteins so that I can look by western blot at my protein of
interest ?

Thank in advance,

Nath