IPTG induction in non E.coli strains

[Aawara Chowdhury]

In <56g48hF291qo1U1 from mid.individual.net>,
 Kyle Legate <legatek from hotmail.com> wrote:

> newsnet customer wrote:
>>> The operator overlaps with the promoter ....  that's how the
>>> repressor prevents RNAP from binding the promoter.  But don't let
>>> facts come in the way of your ignorance.
>> 
>> No. The lacI repressor overlaps the promoter when bound to the operator 
>> thereby preventing RNAP from binding.
>> 
>> ST
>> 
> Oh my, I created a monster! Thanks for the entertainment, but the NCBI 
> nucleotide database considers the operator part of the promoter.
>
> http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=146580

Off course it is.  The operator binding site extends to about "-6" in the
promoter.  There are experiments published by Don Crothers about 25 years
ago in Nature indicating that insertions moving the operator binding site
to start at "+1" allows both RNApol and lacI to footprint at the same time.

What we've encountered is an immature troll who didn't like being told 
that IPTG doesn't bind DNA.  

AC
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