long PCR

[Mandeep Sharma]

HELLO,

I am trying to amplify a 10kb maize genomic DNA fragment using long PCR.
I have already tried Pfu ultra fusion from promega and geneamp high
fidelity PCR system from applied biosystems and they didn't work. If you
have tried amplification of this size fragment and you got the
amplification then can you please suggest which taq polymerase should I
use and what will be the most suitable conditions for long PCR with that
taq?

 

Thanks.

Mandeep

mxs781 from psu.edu