Plasmid construction

[Christian Praetorius]

"Yvonne Couch" <yvonne.couch from dpag.ox.ac.uk> wrote:

>problem is the only way I can think of taking the loops out is by digesting
>and then running a gel and extracting but this results in tiny amounts of
>DNA that fail to ligate under any conditions.  

Why don't you use PCR with primers, which contain the restriction
sites at their ends? Be careful if you do so, since some restriction
enzymes need some additional bases at the end to work properly.

Christian

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X-no-Sig: yes