Southern Blot

[Aawara Chowdhury]

In <drt5i.126$wR3.40 from newsfe03.lga>,
 DK <dk from no.email.thankstospam.net> wrote:

> In article <5blqrsF2tkoopU1 from mid.dfncis.de>, "Anne" <Anne.Roessger from klinik.uni-regensburg.de> wrote:
>>
>>Thank you Peter for your help.
>>I´ll try to find the sequences next to my sensor then by RACE or 
>>restriction-site PCR. I try to find the integration site of my used 
>>transposon. If anybody does know another method to find the integration 
>>sites it would be very helpful for me.
>
> Why not just prime off your transposon and sequence? 
> What am I missing? 

Or, if you really want to clone the transposon insertion site(s), make use of
inverse PCR, which is typically how transposon (or retrovirus) insertion sites
are cloned ...
<http://www.pcrlinks.com/variants/inverse_pcr.htm>

AC
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