in-frame cloning

Pragnya Das via methods%40net.bio.net (by drpragnyadas from gmail.com)
Fri May 25 12:03:18 EST 2007

Previous message: Good Sites in pUC-type vectors for inserting fragments?
Next message: Position wanted in molecular genetics or bioinformatics
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

Hi

can anyone suggest what "in-frame cloning" means giving me a simple example.
I want to subclone an inser in pCDNA3 to RCAS (A) by adding ClaI sites to
either end. Do I have to worry about keeping the insert "in-frame" in the
RCAS (a viral) vector?

Thanks

Pragnya Das

Previous message: Good Sites in pUC-type vectors for inserting fragments?
Next message: Position wanted in molecular genetics or bioinformatics
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

More information about the Methods mailing list