Acid Phenol/chloroform

[Jose de las Heras]

"Dr. Hiranya S. Roychowdhury" <hroychow from nmsu.edu> wrote in message 
news:mailman.535.1194542935.23109.methods from net.bio.net...
> The pH of the acid phenol is usually kept around 4.0.
> We used to make it in lab with Tri.HCl, but Sigma sells the stuff for so
> cheap that it doesn't pay to even equilibrate your own.  Sigma sells both
> Phenol and Phenol Chloroform with pH8.0 or pH4 buffers.

same here, unless you need industrial amounts, the (little) extra expense is 
worth it to avoid hassle/handling.

Jose