protein concentration and desalting

[Ednot]

On Sep 7, 10:02 am, Esmaeil Sadroddiny <sadresm2... from yahoo.com> wrote:
> I am trying to concentrate low amount of protein in solution. In parallel protein sample should be salt free as much as possible. who can introduce me an efficient method?
>   thanks
>
> Sadroddiny.E
>
> ---------------------------------
> Pinpoint customers who are looking for what you sell.

Hello

You can also try a Microcoon Catridge from Millipore, this cartridge
has a molecular cutoff , expressed in kda, it works like a filter
using a centrifuge

Besides , you can add multiples volumes of your sample in the same
sample, and desalt adding a buffer withou salt and concentrate in the
final step.

I use this cartridges  because they have very low non specific binding
of proteins and DNA.

Good luck with your experiment.

Eduardo