Protocol: Inner and Outre membrane Isolation?!

[Natalia Oliveira]

Hello, I was wondering if anyone has a good protocol for inner and outer membrane isolation. I need to detect a Histaged protein on the inner  membrane. I have an outer membrane protein preparation protocol, but not an inner membrane isolation protocol. 
 I know  also that it involves French press and the alternative could be Sonication. I don't have a French press available at the lab, but there is a sonicator, however I don't have a protocol for it. Does any one have of or know where to get one?
  At the same time, does anyone have other suggestion?
   
  Thank you ver much
   
  Natalia

       
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