Page gel

[Jayakumar, R]

That is why we have cooooooling circulating water baths to run our
laaaaaaaaaarge SDS PAGE gels.  
Jay 

-----Original Message-----
From: methods-bounces from oat.bio.indiana.edu
[mailto:methods-bounces from oat.bio.indiana.edu] On Behalf Of yan sun
Sent: Saturday, April 26, 2008 7:39 PM
To: Methods from magpie.bio.indiana.edu
Subject: Page gel

Dear all,
I got a question about PAGE electrophoresis gel to confirm.
When we mention that proper volts condition for running a gel is around
20V/cm.
For eample, my gel is 20 cm, which means that proper Volts is ~400V? Am
I right?
If the volts is toooo high, the gel might toooo hot to get good
resolution, right?
Thx a lot!!!!

Frances
--
Y. F.S.
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