DNA binding protein purification

Itisam Sarangi via methods%40net.bio.net (by itisam.sarangi from gmail.com)
Mon Apr 28 12:13:37 EST 2008

Previous message: Methods Digest, Vol 35, Issue 4
Next message: Old school 2D gel electrophoresis
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

Dear all
I am a regular reader of the methods list. presently I am trying to
purifying a DNA binding protein from E.coli..and trying to remove the
contaminating DNA...
I am using DNAse to remove the contaminating DNA ....but the protein is
precipitaing in the contact with diavalent cations used for DNAse
activity...if any one have  suggestions plz feel free to share....


itisam

Previous message: Methods Digest, Vol 35, Issue 4
Next message: Old school 2D gel electrophoresis
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

More information about the Methods mailing list