Absorbance/ Fluorescence for detection of N-formyl-hydroxy amino acids

Jose Olucha via methods%40net.bio.net (by joseolucha from gmail.com)
Wed Apr 30 22:27:52 EST 2008


Dear all,


Does anyone know of a good method for detecting (I'm thinking absorbance /
fluorescence) N-formyl-hydroxyamino acids (eg. N-formyl-hydroxy-lysine)? If
not, a removal of the formyl group that doesn't involve boiling highly
concentrated acids? Single amino acid deformylases? Have searched literature
to no avail....


A positive answer would make a grad student's life so much better!!

Regards,

Jose


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