Calculating Protein Concentration
StewJW
via methods%40net.bio.net
(by stewjw from gmail.com)
Fri Jan 18 04:02:34 EST 2008
On 17 Jan, 12:46, "Yvonne Couch" <yvonne.co... from dpag.ox.ac.uk> wrote:
> I have two proteins of different molecular weights, how do I calculate the
> molarity of any solution I dissolve them in? For example Protein 1 is 15kDa
> and 1mg is dissolved in 1ml of water, how do I calculate how many moles this
> is?
so 1 mg / ml or 0.001 g / ml
0.001 / 15 000 = 66.66 nmol /ml (66.66 x 10^-9 mole / ml)
or 66.66 x 1000 = 66.66 µmol / L or 66.66 µM (M = Molar = mol / L)
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