elution of co-IP from protein G beads
AK
via methods%40net.bio.net
(by akhan357 from sbcglobal.net)
Wed May 28 12:21:29 EST 2008
Dear experts, I am doing a co-immunoprecipitation experiment. I pull down
protein X with a mAb and detect co IP of protein Y with pAb. protein Y is
heat sensitive and if I heat (~70C) the sample to elute immune complex, I
can not detect protein Y. without heating, protein-G beads do not let the
IgG chains loose, at least. Are there other ways to elute immune complex
without heating? of course low pH is one obvious strategy that I have not
tried yet. thank you in advance.
AK
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