From mail from donnamacdonald.com Sat Dec 1 06:14:50 2007 From: mail from donnamacdonald.com (Donna Macdonald) Date: Mon Dec 3 11:26:50 2007 Subject: [Mycology] Query from Alba Italy Message-ID: My name is Donna Macdonald I am an American living outside of Alba in the Piedmont region in northern Italy. I have been asked to present a synopsis of the American Truffle market, the natural species as well as the cultivated farm varieties for an upcoming Fiera del Tartufi International symposium. I came across your name during my research. I hope I might be possible to send you a few questions that might help my understanding of this subject. Sincerely, Donna Donna Macdonald mail@donnamacdonald.com http://www.donnamacdonald.com +39 333 900 8947 (+ 9 hours from West Coast, + 6 hours from East Coast) From fmrhoades from comcast.net Wed Dec 12 14:34:14 2007 From: fmrhoades from comcast.net (Fred M. Rhoades) Date: Wed Dec 12 15:39:22 2007 Subject: [Mycology] Culturing Rhizopus or other Zygomycota Message-ID: <7.0.1.0.2.20071212110832.03c4fdd8@cc.wwu.edu> I have a set of questions that I have been wondering about for many years. What do folks do for presenting the Zygomycota to introductory biology college students? Which culture source(s) work best? (which genus and which supply firm) Anyone use Mucor? Which agar medium? For many years we have been depending on biological supply house cultures of mating strains (+/-) of Rhizopus stolonifer. On PDA, these cultures always produce an overabundance of asexual sporangia and the zygosporangia are mixed in with the aerial hyphae and asexual sporangia, not arranged in a tidy row between the two strains (as I remember from my first mycology course long ago - but maybe that wasn't Rhizopus). We have tried Phycomyces but the asexual sporangium production is skimpy, and the protective antler-like structures surrounding the zygosporangia interfere with true understanding of the sexual reproductive process. Our lab preparator has expressed some concern about the use of Mucor, because of its association with respiratory diseases. Fred M. Rhoades, Biology Western Washington University Bellingham, WA From ascolocu from zeus.eonet.ne.jp Wed Dec 12 16:23:14 2007 From: ascolocu from zeus.eonet.ne.jp (N.Amano) Date: Thu Dec 13 00:14:16 2007 Subject: [Mycology] Culturing Rhizopus or other Zygomycota In-Reply-To: <7.0.1.0.2.20071212110832.03c4fdd8@cc.wwu.edu> References: <7.0.1.0.2.20071212110832.03c4fdd8@cc.wwu.edu> Message-ID: <20071213062314dr!euq@zeus.eonet.ne.jp> Dear Fred, This book can help you. Author:O ? Donnell, Kerry L. Title :Zygomycetes in culture / Kerry L. O ? Donnell. Published:Athens : Dept. of Botany, University of Georgia, c1979. Sincerely, Nori From jtymo from allstate.com Fri Dec 14 10:44:53 2007 From: jtymo from allstate.com (astrolog) Date: Fri Dec 14 11:39:38 2007 Subject: [Mycology] Sparassis crispa, In-Reply-To: <20070127002959Z6520912-27549+191310@ps12.test.onet.pl> References: <20070127002959Z6520912-27549+191310@ps12.test.onet.pl> Message-ID: <14338258.post@talk.nabble.com> That's me again. One year later. I have Sparassis Crispa strain and cultures to sell or change with other fungis. I develop very effective nutrigent and I'm able to cultivate pure strain. Now I preparing to second stage: I'm working on good basis for cultivating S. Crispa in bags. I use pine sawdust with 3 other major addictions. -- View this message in context: http://www.nabble.com/Sparassis-crispa%2C-tp8661449p14338258.html Sent from the Bio.net - Mycology mailing list archive at Nabble.com. From dmaxwell from pingry.org Mon Dec 17 06:59:59 2007 From: dmaxwell from pingry.org (DAVID L. MAXWELL) Date: Mon Dec 17 14:35:12 2007 Subject: [Mycology] Laminar Flow Hood for Under $100 Message-ID: In the archives, I found a reference to an article by Jim Caldwell about how to build a laminar flow hood for under $100. Does anyone have the text of the article or know where I could get it. Thanks. -David Maxwell The Pingry School Martinsville, NJ From ascolocu from zeus.eonet.ne.jp Mon Dec 17 10:19:02 2007 From: ascolocu from zeus.eonet.ne.jp (N.Amano) Date: Mon Dec 17 14:35:17 2007 Subject: [Mycology] Publications: suitable cultures for zygospore and sporangium demonstration In-Reply-To: <7.0.1.0.2.20071213073758.03c8a6c0@cc.wwu.edu> References: <7.0.1.0.2.20071213073758.03c8a6c0@cc.wwu.edu> Message-ID: <20071218001902$MihmZ@zeus.eonet.ne.jp> Dear Fred, >From these publications you can get enough information on the suitable cultures to demonstrate the zygospore and the sporangium. Studies in Mycology on Line http://www.cbs.knaw.nl/publications/index.htm SIM 4: A study on variability in Mucor hiemalis and related species. M. A. A. SCHIPPER: 40 pp., 1973. SIM 10: On Mucor mucedo, Mucor flavus and related species. M. A. A. SCHIPPER: 33 pp., 1975. SIM 12: On Mucor circinelloides, Mucor racemosus and related species. M. A. A. SCHIPPER: 40 pp., 1976. SIM 17: (1). On certain species of Mucor with a key to all accepted species. (2). On the genera Rhizomucor and Parasitella. M. A. A. SCHIPPER: 70 pp., 1978. SIM 25: A revision of the genus Rhizopus M. A. A. SCHIPPER and J. A. STALPERS: 34 pp., 1984. Sincerely yors, Nori From kent.hoover from gmail.com Tue Dec 18 13:35:28 2007 From: kent.hoover from gmail.com (Ken Hoover) Date: Tue Dec 18 15:18:23 2007 Subject: [Mycology] (no subject) Message-ID: <1fb7b21a0712181035i2e0f77a2ndd85a65e1a63d3b2@mail.gmail.com> In <3qvls2$m6r at ixnews3.ix.netcom.com> jimcald at ix.netcom.com (James Caldwell) writes: > >I just finished an article for a beer magazine on how to build a >laminar flow hood (for about 100 bucks). Since I got the idea >originally from Stamet's book on mushroom culturing, it occurred to me >that some readers of this group might be interested as well. I'll fax >or snail mail copies to any interested party. Wow! I grossly underestimated the interest in this article; I got more than 20 requests and 24hours haven't even elapsed. I have been E-mailing a text version of the article to those who have requested it. If you desire the article with photos and simple drawings (not really necessary IMHO) please send me a SASE at Jim Caldwell 9180 Nana Russell Rd. Owings, MD 20736 (I'll spring for the postage for non-US addresses if you send a self-addressed envelope) In the meantime, requests for the article text or questions can be directed to me at jimcald at ix.netcom.com For the curious, the key to my design was the use of an inexpensive high output (520 cfm) radial output fan with back-curved impellers, and downsizing the HEPA filter to 12" x 24". Going to a larger filter (which the fan will handle) would up the cost from about $100 to $140 or so. Sources for the above parts are included in the article. From Ann.Baldwin from metmuseum.org Tue Dec 18 15:50:41 2007 From: Ann.Baldwin from metmuseum.org (Baldwin, Ann M.) Date: Tue Dec 18 17:30:39 2007 Subject: [Mycology] Laminar Flow Hood Message-ID: Dear Jim Caldwell, I am interested in learning about the low cost laminar flow hood buzzing through the mycology site. I will be happy receiving a text version at ann.baldwin@metmuseum.org, and can receive a fax at 212-650-2192. Many thanks, and happy holidays. Ann Ann M. Baldwin Associate Paper Conservator Metropolitan Museum of Art 1000 Fifth Avenue New York, New York 10028 212-650-2165 From mafarka from email.it Wed Dec 19 03:29:17 2007 From: mafarka from email.it (mfk) Date: Wed Dec 19 14:51:40 2007 Subject: [Mycology] Bioluminescent mushrooms. Message-ID: <4768d66a$0$10624$4fafbaef@reader2.news.tin.it> Hello, i'm looking for bioluminescent mushrooms cultures for free (i'd like to pay just the postage). Anyone can be so gentle to contact me at mafarka@email.it ? Thanks, F. From mycos from shaw.ca Wed Dec 19 13:54:18 2007 From: mycos from shaw.ca (Mycos) Date: Wed Dec 19 14:51:54 2007 Subject: [Mycology] (no subject) In-Reply-To: <1fb7b21a0712181035i2e0f77a2ndd85a65e1a63d3b2@mail.gmail.com> References: <1fb7b21a0712181035i2e0f77a2ndd85a65e1a63d3b2@mail.gmail.com> Message-ID: <476968DA.8050802@shaw.ca> I was wondering what the trick had to have been! Many years ago Paul Kroeger picked up a second hand one from the UBC auction for a mere $100. Needless to say, both he and I were pleased as punch considering the filter alone was worth more than that --- nevertheless an assembled, ready-to-go LFH. And they are indeed the cat's meow for culture work, but surprisingly not as commonplace as one would expect. For instance, some time ago I went up to the local college here in Kamloops to do some private work which they had graciously loaned their facilities to me for. When I got there however I find they were using their fume hoods as a kind of poor-man's glove-box. Apart from my embarrassment at having to decline the offer (I decided the cost of my specimens being lost to contamination wasn't worth the risk of me being gracious and accepting the circumstances) was the curious matter of a mycologist being the acting Dean of the Science/Forestry wing at the time. I would have thought that LFHs would have been his first order of business! Oh, well. The social-sciences need their specimens as well . Gary Williams Ken Hoover wrote: > In <3qvls2$m6r at ixnews3.ix.netcom.com> jimcald at ix.netcom.com (James > Caldwell) writes: >> I just finished an article for a beer magazine on how to build a >> laminar flow hood (for about 100 bucks). Since I got the idea >> originally from Stamet's book on mushroom culturing, it occurred to me >> that some readers of this group might be interested as well. I'll fax >> or snail mail copies to any interested party. > > > Wow! I grossly underestimated the interest in this article; I got > more than 20 requests and 24hours haven't even elapsed. I have been > E-mailing a text version of the article to those who have requested it. > If you desire the article with photos and simple drawings (not really > necessary IMHO) please send me a SASE at > > Jim Caldwell > 9180 Nana Russell Rd. > Owings, MD 20736 > > (I'll spring for the postage for non-US addresses if you send a > self-addressed envelope) > > In the meantime, requests for the article text or questions can be > directed to me at jimcald at ix.netcom.com > > For the curious, the key to my design was the use of an inexpensive > high output (520 cfm) radial output fan with back-curved impellers, and > downsizing the HEPA filter to 12" x 24". Going to a larger filter > (which the fan will handle) would up the cost from about $100 to $140 > or so. Sources for the above parts are included in the article. > > _______________________________________________ > Mycology mailing list > Mycology@net.bio.net > http://www.bio.net/biomail/listinfo/mycology > From mafarka from email.it Thu Dec 20 03:43:52 2007 From: mafarka from email.it (mfk) Date: Thu Dec 20 11:25:49 2007 Subject: [Mycology] (no subject) In-Reply-To: References: <1fb7b21a0712181035i2e0f77a2ndd85a65e1a63d3b2@mail.gmail.com> Message-ID: <476a2b54$0$36447$4fafbaef@reader5.news.tin.it> Mycos ha scritto: > I was wondering what the trick had to have been! Many years ago Paul > Kroeger picked up a second hand one from the UBC auction for a mere > $100. Needless to say, both he and I were pleased as punch considering > the filter alone was worth more than that --- nevertheless an assembled, > ready-to-go LFH. > > And they are indeed the cat's meow for culture work, but surprisingly > not as commonplace as one would expect. For instance, some time ago I > went up to the local college here in Kamloops to do some private work > which they had graciously loaned their facilities to me for. When I got > there however I find they were using their fume hoods as a kind of > poor-man's glove-box. > > Apart from my embarrassment at having to decline the offer (I decided > the cost of my specimens being lost to contamination wasn't worth the > risk of me being gracious and accepting the circumstances) was the > curious matter of a mycologist being the acting Dean of the > Science/Forestry wing at the time. I would have thought that LFHs would > have been his first order of business! Oh, well. The social-sciences > need their specimens as well . > > Gary Williams > > Ken Hoover wrote: >> In <3qvls2$m6r at ixnews3.ix.netcom.com> jimcald at ix.netcom.com (James >> Caldwell) writes: >>> I just finished an article for a beer magazine on how to build a >>> laminar flow hood (for about 100 bucks). Since I got the idea >>> originally from Stamet's book on mushroom culturing, it occurred to me >>> that some readers of this group might be interested as well. I'll fax >>> or snail mail copies to any interested party. >> >> >> Wow! I grossly underestimated the interest in this article; I got >> more than 20 requests and 24hours haven't even elapsed. I have been >> E-mailing a text version of the article to those who have requested it. >> If you desire the article with photos and simple drawings (not really >> necessary IMHO) please send me a SASE at >> >> Jim Caldwell >> 9180 Nana Russell Rd. >> Owings, MD 20736 >> >> (I'll spring for the postage for non-US addresses if you send a >> self-addressed envelope) >> >> In the meantime, requests for the article text or questions can be >> directed to me at jimcald at ix.netcom.com >> >> For the curious, the key to my design was the use of an inexpensive >> high output (520 cfm) radial output fan with back-curved impellers, and >> downsizing the HEPA filter to 12" x 24". Going to a larger filter >> (which the fan will handle) would up the cost from about $100 to $140 >> or so. Sources for the above parts are included in the article. >> >> _______________________________________________ >> Mycology mailing list >> Mycology@net.bio.net >> http://www.bio.net/biomail/listinfo/mycology >> > I can understand you! However gloveboxes work perfectly, and in case of failure you can always clean the culture with a couple of transfers (that are necessary in a glovebox if molds are present, as you know). A lot of people understimate the power of a cheap glovebox made of a common plastic container with 2 holes. However flowhood is a must, to work more confortable and on a higher rate of success. I've read of people that clone mushrooms in loco using a disposable plastic bag with 2 holes for the hand and a spray like alcohol. Fabio - www.FreeSporePrints.org From mafarka from email.it Thu Dec 20 04:03:52 2007 From: mafarka from email.it (mfk) Date: Thu Dec 20 11:25:55 2007 Subject: [Mycology] Sparassis crispa, In-Reply-To: References: <20070127002959Z6520912-27549+191310@ps12.test.onet.pl> Message-ID: <476a3006$0$36442$4fafbaef@reader5.news.tin.it> astrolog ha scritto: > That's me again. One year later. I have Sparassis Crispa strain and cultures > to sell or change with other fungis. I develop very effective nutrigent and > I'm able to cultivate pure strain. > Now I preparing to second stage: I'm working on good basis for cultivating > S. Crispa in bags. I use pine sawdust with 3 other major addictions. Hello astrolog, received my email? I'm inetrested in Sparassis crispa! Thanks, Fabio - www.FreeSporePrints.org