We culture neurons derived from the NTera-2 cell line. 10 ug/ml poly-d-
lysine is used in Borate Buffer: 77 mM boric acid, 6.7 mM Na2B4O7 (Borax),
pH 8.4. We coat sterile glass discs in this solution and leave them
covered overnight in the hood. The following morning we aspirate off the
solution/unbound poly and leave them uncovered to dry completely (2 hrs).
We then coat with Matrigel (1:36 in DMEM; Collaborative Research) for 2
hrs - in the incubator, aspirate that off, and plate neurons. This works
very well.
Good Luck, Michelle LaPlaca
