In article <ladic.366.0021B5A9 at cs.ubc.ca> Lance Ladic, ladic at cs.ubc.ca
writes:
>If anyone who reads this newsgroup has used CT-HRP as a retrograde
tracer, I
>would very much like to hear from you.
>>Also, if anyone knows of a source that supplies commercially
manufactured
>CT-HRP and antibodies to the complex (anti-CT or anti-HRP?, preferrably
not
>raised in rabbit) please let me know. While I know that the conjugated
tracer
>complex can be visualized via an enzymatic reaction for HRP, I would
like to
>see if I can use the tracer for some multi-label fluorescence work. I
have
>checked the (few) catalogues around my Lab and have had little luck
finding
The place to buy cholera toxin products is:
List Biological Laboratories, Inc.
501-B Vandell Way, Campbell, California
95008-6967 U.S.A.
(408) 866-6363
(800) 726-3213
FAX: (408) 866-6364
They sell the cholera toxin subunit B for retrograde tracing and a
goat polyclonal antibody against it. They also sell the toxin conjugated
to HRP,
or biotin, or colloidal gold,or several of the more popular fluorochromes.
I personally favor the colloidal gold conjugated tracer, as it can be
visualized with
a simple silver intensification. Hope this helps.
Jamie Boyd
Dept. of Ophthalmology
University of British Columbia
Boyd at org.ecc.ubc.ca
