IUBio

TUNELing in the brain

Edward Mills tedmills at sage.cc.purdue.edu
Tue Dec 6 15:48:17 EST 1994


jerikse at news.luc.edu (Jason L. Eriksen) writes:

>If you have used the TUNEL technique or a variant thereof to look at apoptosis
>in neurons, I would like to hear from you.  Our lab is just beginning to use 
>this technique, and any advice you can give will be greatly appreciated. 

>	Jason Eriksen
In a recent Biochemica (journal) article (I can't recall the issue #) of
2 to 3 months ago there was a great survey article on different methods
of apoptosis detection.  Hope that helps.  In our lab we do neuronal
apoptosis and detect it with the all too inconvenient DNA ladders. 
This assay is cumbersome with low density cells.

I'd be interested to hear what you're thinking!! 

Ted





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