I have recently begun working with 2 neuroblastoma cell lines (LA-N-2 and
SK-N-MC) and have been attempting to differentiate them with retinoic
acid. After approx. 1 week of treating the cells, I have seen no
alterations in growth of the cells nor have I detected any neurite
outgrowth. I dissolved the retinoic acid (RA) in 100% ethanol and treat the
cells every day with 10 microM RA or 0.1% ethanol vol/vol (control). Any
suggestions on modifications to my protocol or alternative protocols
would be greatly appreciated.
Thanks, Christopher Chambers
