I just realized that the 50 mMolar HEPES in which we make our membrane
preps is interfering with our modified Lowrey protein determinations. How
do you get around that problem? I found nothing in the literature after
the 1975 Anal.Biochem 67, p198 notation that it and TRIS and some other
buffers do interfer. Hope you can help.
Thanks,
Bob Perigo bperigo at u.washington.edu
