On 19 Dec 1994 11:31:00 -0800,
Fiberman <william at neuro.usc.edu> wrote:
>Hello Netters,
>>I wish to detect a receptor at the synapses of the mammalian brain. I want to
>separate receptors at the synapse from those inside the cell (ie. those in the
>ER, Golgi, or microsomes). If you know of a reference that talks about
>purifying synaptic proteins, please let me know. Thanks!
>>-fm
Any general biochemistry cookbook has a recipe for isolating synaptosomes.
However, hear is a couple of references to a reasonable procedure. Neuron
9:929-942, 1992. PNAS 91:3954-3958, 1994.
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