>>Okay bionetters, you were so helpful in helping me find a neuroscience
>>textbook that I am going back to the well. This time, I am looking for
>>someone to recommend a neuroscience lab techniques book. I am hoping to
>>find something that is fairly extensive and understandable. Any
>>recommendations?
>>>>Sean P. Sullivan
>>ssulliva at opal.tufts.edu>>
>For a comprehensive treatment of electrophysiological techniques try:
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>Single Channel Recording (B. Sakmann & E. Neher, eds), Plenum, 1983
This book is not suited as a practical lab guide. Chapter 1 is how
to select components to build a patch clamp amplifier. The
chapters on data analysis are well grounded in theoretical
considerations but difficult to wade through. Remember this was
written early in the development of patch clamp techniques and lab
techniques described in the chapters have changed since then with
advances in micromanipulators, perfusion exchange apparatus, etc.
The new edition, due out soon, is better but still not a lab book.
A better book for methods ion channel reseach is Methods in
Enzymology vol 207. It about $100 without discounts but well
worth borrowing and reading. If you are serious about setting up
a patch clamp lab, it is worth buying.
However, the best reference for a lab set up is to go to a
laboratory and talk to the people who are doing the work. They
can tell you what works and make suggestions on how to improve the
set up. Everyone has modifications they would like to do to make
work easier but do not have time to implement.
[delete]
>Carlo
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Who was that Masked Interrupt?
jmwright at helix.nih.gov Jerry M. Wright
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